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The PureLink HQ Mini Plasmid Purification Kit is designed for the isolation of high-quality plasmid DNA that is suitable for restriction enzyme digestion, PCR, sequencing, bacterial cell transformation, and mammalian cell transfection. With the kit, plasmid DNA can be isolated from varying numbers of bacterial cells.

The PureLink HQ Mini Plasmid Purification Kit has the following benefits:

  • Higher spinning column capacity for plasmid DNA compared to other commercially available plasmid purification systems
  • Designed to isolate high-quality plasmid DNA in less than an hour
  • Minimal contamination of genomic DNA from the purified sample
  • Reliable performance of purified DNA in downstream applications

Process overview

Bacterial cells are pelleted, resuspended, and lysed. The lysate is then neutralized and the conditions for subsequent binding are adjusted. After clarification by centrifugation, the lysate is run through the PureLink spin column. The DNA is bound to the silica-based membrane on the column and impurities are removed by a single wash step. The DNA is then eluted in elution buffer or water. For research use only. It should not be used in diagnostic procedures.


Type of end product: Plasmid DNA

For use with (application): PCR, Transfection, Sequencing, Transformation, Cloning

High-performance compatibility: Not compatible with high performance (manual)

No. of reactions: 100 preparations

Preparation scale: <100 µg (small scale) plasmid DNA

Product line: PureLink ™

Shipping condition: Room temperature

Target: Plasmid DNA

Format: Rotating column

Insulation technology: Silica rotating column

Example type: Bacterial culture

Scale: Mini

Content and storage

The PureLink HQ Mini Plasmid DNA Purification Kit includes enough PureLink columns, collection tubes, elution tubes, and reagents for 100 purifications. Store components at room temperature. Store the resuspension buffer after adding RNase A at +4 ° C. All reagents have guaranteed stability of 6 months when stored correctly.