The PureLink HQ Mini Plasmid Purification Kit is designed for the isolation of high-quality plasmid DNA that is suitable for restriction enzyme digestion, PCR, sequencing, bacterial cell transformation, and mammalian cell transfection. With the kit, plasmid DNA can be isolated from varying numbers of bacterial cells.
The PureLink HQ Mini Plasmid Purification Kit has the following benefits:
- Higher spinning column capacity for plasmid DNA compared to other commercially available plasmid purification systems
- Designed to isolate high-quality plasmid DNA in less than an hour
- Minimal contamination of genomic DNA from the purified sample
- Reliable performance of purified DNA in downstream applications
Bacterial cells are pelleted, resuspended, and lysed. The lysate is then neutralized and the conditions for subsequent binding are adjusted. After clarification by centrifugation, the lysate is run through the PureLink spin column. The DNA is bound to the silica-based membrane on the column and impurities are removed by a single wash step. The DNA is then eluted in elution buffer or water. For research use only. It should not be used in diagnostic procedures.
Type of end product: Plasmid DNA
For use with (application): PCR, Transfection, Sequencing, Transformation, Cloning
High-performance compatibility: Not compatible with high performance (manual)
No. of reactions: 100 preparations
Preparation scale: <100 µg (small scale) plasmid DNA
Product line: PureLink ™
Shipping condition: Room temperature
Target: Plasmid DNA
Format: Rotating column
Insulation technology: Silica rotating column
Example type: Bacterial culture
Content and storage
The PureLink HQ Mini Plasmid DNA Purification Kit includes enough PureLink columns, collection tubes, elution tubes, and reagents for 100 purifications. Store components at room temperature. Store the resuspension buffer after adding RNase A at +4 ° C. All reagents have guaranteed stability of 6 months when stored correctly.